FinePure EndoFree Plasmid Maxi Plus Kit (10 Preps)

Recommended bacteria amount for each practice: For high-copy plasmid, it is recommended to use 100 ml of bacteria culture media; For low-copy plasmid, 200 ml of bacteria culture media is recommended.

Features：

1. Unique glass cellulose membrane adsorption technology, efficient and specific binding plasmid DNA;

2. Preparation of Buffer ETR, which can effectively remove more than 99% of endotoxin;

3. The plasmids obtained had good yield and stability.

Tests from Customer:

1. High-copy plasmid pUC 19, extracted from 150 ml bacterial culture using Genfine D919 and competitors, was eluted in 1 ml Buffer TB.

2. Low-copy plasmid extraction

Low-copy plasmid pBR322, extracted from 300 ml bacterial culture using Genfine D919 and competitors, was eluted in 1 ml Buffer TB.

3. pEGFP obtained with Genfine D919 and Competitor T were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by transfection reagent. Expression of GFP was detected 24 hours after transfection

Storage：

EndoFree Plasmid Maxi Plus Kit can be stored at room temperature (15~25) °C under dry conditions for up to 12 months. Add RNase A to Buffer P1 before first use, mix well and store at 2~8 °C. The mixture can be stored stably for more than 6 months.

Required Reagents：

ethanol

Performance:

High-copy plasmid extraction

Downloadable Documents

Genfine D909 Manual-EN