African Swine Fever Virus Real-time PCR Detection Kit utilizes a patented buffer system, which includes nucleic acids extraction buffer 1 and buffer 2 for ASFV directly lysis, ASFV-PCR reaction buffer, ASFV-PCR enzyme, negative control, and positive control. Our product can detect ASFV via RT-PCR from various blood and histopathological samples directly. There is no proteinase digestion, high-temperature incubation, and proteins or other impurities removal steps. And there is no organic solvent extraction or absolute ethanol precipitation. So the experiment t is simple, quick, stable, and reliable. The ASFV-PCR reaction buffer in our kit is a kind of strongly compatible amplification reagent, which can support effective and specific amplification without proteins or impurities being completely removed. This kit has a special reaction buffer, PCR reaction enhancer, and stabilizer. All of these make it simple, quick, stable, highly sensitive, and highly specific, so it can be especially used for the development of clinical molecular diagnostic products.
◈ Simple and fast: with the pre-packaged kit, only sample addition is required before using our GENFINE Purifier Instrument. It can be as fast as 13 min for 96 samples one time；
◈ Suitable for all kinds of complex samples DNA/RNA extraction: whole blood, plasma, saliva, mouth swab, tissue lapping fluid, lymph, internal organs, and so on；
◈ Safe and convenient for processing, high quality, and high yield DNA/RNA.
Part A can keep for 12 months below -20℃; part B can drily keep for 12 months at 15-25℃.